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ProductsBack/Macrophage Inflammatory Protein 3 Beta (MIP3b) Polyclonal Antibody

Macrophage Inflammatory Protein 3 Beta (MIP3b) Polyclonal Antibody

CAU28627
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetCcl19
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Overview

  • Supplier
    Biomatik
  • Product Name
    Macrophage Inflammatory Protein 3 Beta (MIP3b) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID362506
  • Target name
    Ccl19
  • Target description
    C-C motif chemokine ligand 19
  • Target synonyms
    C-C motif chemokine 19, chemokine (C-C motif) ligand 19
  • Host
    Rabbit
  • Protein IDD3ZI84
  • Protein Name
    C-C motif chemokine
  • Scientific Description
    The Macrophage Inflammatory Protein 3 Beta (MIP3b) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (A01605-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysate,Lane 2: rat lung tissue lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat stomach tissue lysate,Lane 5: rat PC-12 whole Cell lysate,Lane 6: mouse thymus tissue lysate,Lane 7: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody (Catalog # A01605-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD.
Product group Antibodies
Boster Bio
Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband(r)A01605-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetCcl19
  • SizePrice