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MAD2B/MAD2L2 Rabbit pAb

A9861
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMAD2L2
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Overview

  • Supplier
    ABclonal
  • Product Name
    MAD2B/MAD2L2 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10459
  • Target name
    MAD2L2
  • Target description
    mitotic arrest deficient 2 like 2
  • Target synonyms
    FANCV, MAD2B, POLZ2, REV7, mitotic spindle assembly checkpoint protein MAD2B, MAD2 (mitotic arrest deficient, yeast, homolog)-like 2, MAD2 mitotic arrest deficient-like 2, MAD2-like protein 2, REV7 homolog, hREV7, mitotic arrest deficient 2-like protein 2, mitotic arrest deficient homolog-like 2, polymerase (DNA-directed), zeta 2, accessory subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UI95
  • Protein Name
    Mitotic spindle assembly checkpoint protein MAD2B
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAD2L2 using anti-MAD2L2 antibody (A02357-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysats. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD2L2 antigen affinity purified polyclonal antibody (Catalog # A02357-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD2L2 at approximately 24 kDa. The expected band size for MAD2L2 is at 24 kDa.
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