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MAFF Antibody

LS-C747990
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMAFF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAFF Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 14kDa/17kDa, while the observed MW by Western blot was 18kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 14kDa/17kDa, while the observed MW by Western blot was 18kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23764
  • Target name
    MAFF
  • Target description
    MAF bZIP transcription factor F
  • Target synonyms
    U-MAF, hMafF, transcription factor MafF, v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAFF using anti-MAFF antibody (A07784-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFF antigen affinity purified polyclonal antibody (Catalog # A07784-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFF at approximately 18 kDa. The expected band size for MAFF is at 18 kDa.
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