Paraformaldehyde-fixed, paraffin embedded human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MAGEB3 Polyclonal Antibody, Unconjugated (bs-6823R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MAGEB3 Polyclonal Antibody, Unconjugated (bs-6823R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
MAGEB3 Polyclonal Antibody
BS-6823R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityEquine, Human, Mouse, Rat
TargetMAGEB3
Overview
- SupplierBioss
- Product NameMAGEB3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID4114
- Target nameMAGEB3
- Target descriptionMAGE family member B3
- Target synonymsCT3.5, melanoma-associated antigen B3, MAGE-B3 antigen, cancer/testis antigen family 3, member 5, melanoma antigen family B, 3, melanoma antigen family B3
- HostRabbit
- IsotypeIgG
- ReactivityEquine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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