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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA013771(MAGEL2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA013771(MAGEL2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: CSB-PA013771(MAGEL2 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes

MAGEL2 Antibody

CSB-PA013771
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMAGEL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAGEL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54551
  • Target name
    MAGEL2
  • Target description
    MAGE family member L2
  • Target synonyms
    MAGE-like protein 2; melanoma antigen family L2; NDNL1; necdin-like protein 1; nM15; protein nM15; PWLS; SHFYNG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJ55
  • Protein Name
    MAGE-like protein 2
  • Scientific Description
    Prader-Willi syndrome (PWS) is caused by the loss of expression of imprinted genes in chromosome 15q11-q13 region. Affected individuals exhibit neonatal hypotonia, developmental delay, and childhood-onset obesity. Necdin (NDN), a gene involved in the terminal differentiation of neurons, localizes to this region of the genome and has been implicated as one of the genes responsible for the etiology of PWS. This gene is structurally similar to NDN, is also localized to the PWS chromosomal region, and is paternally imprinted, suggesting a possible role for it in PWS.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAGEL2 using anti-MAGEL2 antibody (A06305-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGEL2 antigen affinity purified polyclonal antibody (Catalog # A06305-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGEL2 at approximately 133 kDa. The expected band size for MAGEL2 is at 133 kDa.
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