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Malectin antibody detects KIAA0152 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Malectin antibody (GTX102021) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Malectin antibody detects KIAA0152 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Malectin antibody (GTX102021) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Malectin antibody detects KIAA0152 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Malectin antibody (GTX102021) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

Malectin antibody

GTX102021
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMLEC
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Overview

  • Supplier
    GeneTex
  • Product Name
    Malectin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.45 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9761
  • Target name
    MLEC
  • Target description
    malectin
  • Target synonyms
    KIAA0152, malectin, oligosaccharyltransferase complex subunit (non-catalytic)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14165
  • Protein Name
    Malectin
  • Scientific Description
    Carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Malectin/MLEC using anti-Malectin/MLEC antibody (A10455-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Malectin/MLEC antigen affinity purified polyclonal antibody (Catalog # A10455-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Malectin/MLEC at approximately 32 kDa. The expected band size for Malectin/MLEC is at 32 kDa.
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