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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA838784LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA838784LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA838784LA01HU at dilution of 1:100

MAML1 Antibody

CSB-PA838784LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAML1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAML1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9794
  • Target name
    MAML1
  • Target description
    mastermind like transcriptional coactivator 1
  • Target synonyms
    Mam1; Mam-1; mastermind homolog; mastermind-like 1; mastermind-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92585
  • Protein Name
    Mastermind-like protein 1
  • Scientific Description
    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAML1 using anti-MAML1 antibody (A03990-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MOLT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAML1 antigen affinity purified polyclonal antibody (Catalog # A03990-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAML1 at approximately 130 kDa. The expected band size for MAML1 is at 108 kDa.
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Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
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