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ProductsBack/Mannan Binding Lectin (MBL2) Rabbit Polyclonal Antibody
Western blot analysis of lysates from Mouse serum
Western blot analysis of lysates from Mouse serum
Western blot analysis of lysates from Mouse serum

Mannan Binding Lectin (MBL2) Rabbit Polyclonal Antibody

TA332941
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMBL2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Mannan Binding Lectin (MBL2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4153
  • Target name
    MBL2
  • Target description
    mannose binding lectin 2
  • Target synonyms
    COLEC1, HSMBPC, MBL, MBL2D, MBP, MBP-C, MBP1, MBPD, mannose-binding protein C, collectin-1, mannan-binding lectin, mannose-binding lectin (protein C) 2, soluble (opsonic defect), mannose-binding lectin 2, soluble (opsonic defect)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11226
  • Protein Name
    Mannose-binding protein C
  • Scientific Description
    Rabbit anti-MBL2 Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
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