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MAOA Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62029R-BF647
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetMAOA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MAOA Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:500-2000), IF(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID4128
  • Target name
    MAOA
  • Target description
    monoamine oxidase A
  • Target synonyms
    amine oxidase [flavin-containing] A; BRNRS; MAO-A; monoamine oxidase type A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21397
  • Protein Name
    Amine oxidase [flavin-containing] A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAOA using anti-MAOA antibody (PB9664). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOA antigen affinity purified polyclonal antibody (Catalog # PB9664) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOA at approximately 60 kDa. The expected band size for MAOA is at 60 kDa.
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