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Western blot All lanes: MAOB antibody at 9microg/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 47 kDa Observed band size: 59, 27 kDa
Western blot All lanes: MAOB antibody at 9microg/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 47 kDa Observed band size: 59, 27 kDa
Western blot All lanes: MAOB antibody at 9microg/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 47 kDa Observed band size: 59, 27 kDa

MAOB Antibody

CSB-PA013399LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMAOB
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAOB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4129
  • Target name
    MAOB
  • Target description
    monoamine oxidase B
  • Target synonyms
    amine oxidase [flavin-containing] B, MAO, brain, MAO, platelet, MAO-B, adrenalin oxidase, monoamine oxidase type B, tyramine oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27338
  • Protein Name
    Amine oxidase [flavin-containing] B
  • Scientific Description
    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAOB using anti-MAOB antibody (PB9665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (Catalog # PB9665) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
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