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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA578056(MAP1LC3A Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA578056(MAP1LC3A Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA578056(MAP1LC3A Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: x200)

MAP1LC3A Antibody

CSB-PA578056
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAP1LC3A
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP1LC3A Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84557
  • Target name
    MAP1LC3A
  • Target description
    microtubule associated protein 1 light chain 3 alpha
  • Target synonyms
    ATG8E; autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H492
  • Protein Name
    Microtubule-associated proteins 1A/1B light chain 3A
  • Scientific Description
    MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A antigen affinity purified polyclonal antibody (Catalog # A01543-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18KD. The expected band size for MAP1LC3A is at 14KD.
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