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ProductsBack/MAP2K5 / MEK5 Antibody (Preservative Free)

MAP2K5 / MEK5 Antibody (Preservative Free)

LS-C815644
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAP2K5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAP2K5 / MEK5 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:300 - 1:10000), IF (1:300 - 1:10000), IHC-P (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5607
  • Target name
    MAP2K5
  • Target description
    mitogen-activated protein kinase kinase 5
  • Target synonyms
    dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; MEK5; PRKMK5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK5/MAP2K5 using anti-MEK5/MAP2K5 antibody (A03980-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates, Lane 9: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK5/MAP2K5 antigen affinity purified polyclonal antibody (Catalog # A03980-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK5/MAP2K5 at approximately 50KD. The expected band size for MEK5/MAP2K5 is at 50KD.
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