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ProductsBack/MAP2K6 / MEK6 / MKK6 Antibody (clone 3H12B9)

MAP2K6 / MEK6 / MKK6 Antibody (clone 3H12B9)

LS-C758652
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMAP2K6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAP2K6 / MEK6 / MKK6 Antibody (clone 3H12B9)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H12B9
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5608
  • Target name
    MAP2K6
  • Target description
    mitogen-activated protein kinase kinase 6
  • Target synonyms
    dual specificity mitogen-activated protein kinase kinase 6; MAPK/ERK kinase 6; MAPKK 6; MAPKK6; MEK 6; MEK6; MKK6; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 3; SAPKK3; SAPKK-3; stress-activated protein kinase kinase 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
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