Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
MAP3K14 antibody
GTX27204
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameMAP3K14 antibody
- Delivery Days Customer9
- Application Supplier NoteIP: Use at a dilution of 1/500. WB: Use at a dilution of 1/2000. Optimal dilutions/concentrations should be determined by the end user.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Scientific DescriptionNIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK, initially isolated from a human B cell cDNA library, contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to I B phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203