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MAPK13 Polyclonal Antibody

RD70072A
Reddot Biotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMAPK13
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    MAPK13 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5603
  • Target name
    MAPK13
  • Target description
    mitogen-activated protein kinase 13
  • Target synonyms
    MAP kinase 13; MAP kinase p38 delta; MAPK 13; MAPK-13; mitogen-activated protein kinase 13; mitogen-activated protein kinase p38 delta; p38delta; PRKM13; SAPK4; stress-activated protein kinase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13,SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAPK13 using anti-MAPK13 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK13 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK13 at approximately 42 kDa. The expected band size for MAPK13 is at 42 kDa.
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