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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA620890LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA620890LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA620890LA01HU at dilution of 1:100

MAPRE1 Antibody

CSB-PA620890LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAPRE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAPRE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22919
  • Target name
    MAPRE1
  • Target description
    microtubule associated protein RP/EB family member 1
  • Target synonyms
    adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; end-binding protein 1; microtubule-associated protein RP/EB family member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15691
  • Protein Name
    Microtubule-associated protein RP/EB family member 1
  • Scientific Description
    Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EB1/MAPRE1 using anti-EB1/MAPRE1 antibody (A02070-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Ramos whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EB1/MAPRE1 antigen affinity purified polyclonal antibody (Catalog # A02070-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EB1/MAPRE1 at approximately 35 kDa. The expected band size for EB1/MAPRE1 is at 30 kDa.
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