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Western blot analysis of extracts of various cell lines using MARS Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using MARS Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using MARS Polyclonal Antibody at 1:1000 dilution.

MARS Polyclonal Antibody

E-AB-62822
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    MARS Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MARS1 using anti-MARS1 antibody (A00216-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARS1 antigen affinity purified polyclonal antibody (Catalog # A00216-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARS1 at approximately 99 kDa. The expected band size for MARS1 is at 101 kDa.
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Product group Antibodies
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ReactivityHuman
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Product group Antibodies
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Product group Antibodies
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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA004125 antibody. Corresponding MARS RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
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Product group Antibodies
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Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal (GTX109864) diluted at 1:1000.
Product group Antibodies
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MARS antibody [N3C2], InternalGTX109864
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