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WB analysis of various sample lysates using GTX55696 MASPIN antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55696 MASPIN antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55696 MASPIN antibody. Dilution : 1:1000 Loading : 25microg per lane

MASPIN antibody

GTX55696
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSERPINB5
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Overview

  • Supplier
    GeneTex
  • Product Name
    MASPIN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5268
  • Target name
    SERPINB5
  • Target description
    serpin family B member 5
  • Target synonyms
    PI5, maspin, serpin B5, PI-5, peptidase inhibitor 5, protease inhibitor 5 (maspin), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5, serpin peptidase inhibitor, clade B (ovalbumin), member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36952
  • Protein Name
    Serpin B5
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MASPIN/SERPINB5 using anti-MASPIN/SERPINB5 antibody (PB9356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN/SERPINB5 antigen affinity purified polyclonal antibody (Catalog # PB9356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASPIN/SERPINB5 at approximately 42 kDa. The expected band size for MASPIN/SERPINB5 is at 42 kDa.
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