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ProductsBack/Mast Cell Tryptase Recombinant Antibody
Mast Cell lysate probed with Mast Cell Tryptase (3G3) Monoclonal Antibody (bsm-52533R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mast Cell lysate probed with Mast Cell Tryptase (3G3) Monoclonal Antibody (bsm-52533R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mast Cell lysate probed with Mast Cell Tryptase (3G3) Monoclonal Antibody (bsm-52533R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Mast Cell Tryptase Recombinant Antibody

BSM-52533R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTPSAB1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Mast Cell Tryptase (3G3) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7177
  • Target name
    TPSAB1
  • Target description
    tryptase alpha/beta 1
  • Target synonyms
    epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; TPS1; TPS2; TPSB1; TPSB2; tryptase alpha II; tryptase alpha/beta-1; tryptase alpha-1; tryptase beta I; tryptase beta-1; Tryptase beta-2; Tryptase II; tryptase-1; Tryptase-2; tryptase-I; tryptase-III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15661
  • Protein Name
    Tryptase alpha/beta-1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mast Cell Tryptase antigen affinity purified polyclonal antibody (Catalog # PB10016) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mast Cell Tryptase at approximately 30 kDa. The expected band size for Mast Cell Tryptase is at 31 kDa.
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