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Immunohistochemical analysis of paraffin-embedded human tonsil, using PD1 Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded mouse lung, using Caveolin-1 Antibody.
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human liver cancer, using Bcl-XL Antibody(M00181-1) BCL2L1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2L1 Antibody (M00181-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of NCAM expression in SH-SY5Y cell lysate (M00184-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 monoclonal antibody (Catalog # M00184-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRAF6 Antibody(M00185-1) TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185-1)overnight at 4°C Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TRAF6 Antibody(M00185) TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Western blot analysis of MUC1 expression in T47 D cell lysate.
Product group Antibodies
Boster Bio
Western blot analysis of Argonaute 2 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
Western blot analysis of FMRP expression in K562 cell lysate.
Product group Antibodies
Boster Bio
Western blot analysis of CD38 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio