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Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1-PE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Figure 1. Western blot analysis of PTPN12 using anti-PTPN12 antibody (A02762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN12 antigen affinity purified polyclonal antibody (Catalog # A02762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN12 at approximately 110 kDa. The expected band size for PTPN12 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-PTPN12 Antibody Picoband(r)A02762-1
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPTPN12
  • SizePrice
Product group Antibodies
Boster Bio
Anti-PTN12 PTPN12 AntibodyA02762
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetPTPN12
  • SizePrice
1213141516

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