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Immunohistochemical analysis of paraffin-embedded human pancreas, using PDX1 Antibody.
Product group Antibodies
Boster Bio
Anti-PDX1/Ipf1 Rabbit Monoclonal AntibodyM00491
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPDX1
Western blot analysis of SRSF1 expression in mouse spleen lysate using anti-SRSF1 antibody (M00497). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF1 antigen affinity purified polyclonal antibody (Catalog # M00497) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SRSF1.
Product group Antibodies
Boster Bio
Anti-SF2 Monoclonal AntibodyM00497
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSRSF1
Western blot analysis of Cytochrome P450 2D6 expression in K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-Cytochrome P450 2D6 CYP2D6 Monoclonal AntibodyM00498
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCYP2D6
Western blot analysis of GATA4 expression in HeLa cell lysate (M00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 monoclonal antibody (Catalog # M00499-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4
Product group Antibodies
References
Boster Bio
Anti-GATA4 Rabbit Monoclonal AntibodyM00499-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetGATA4
Western blot analysis of SNF5 in (1) HeLa cell lysate; (2) K562 cell lysate (M00500). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCB1 monoclonal antibody (Catalog # M00500) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCB1
Product group Antibodies
Boster Bio
Anti-SNF5 SMARCB1 Rabbit Monoclonal AntibodyM00500
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetSMARCB1
Western blot analysis of Filamin A expression in (1) NIH/3T3 cell lysate;(2) HeLa cell lysate (M00502). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLNA monoclonal antibody (Catalog # M00502) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLNA
Product group Antibodies
Boster Bio
Anti-Filamin A FLNA Rabbit Monoclonal AntibodyM00502
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFLNA
Western blot analysis of GR expression in A431 cell lysate.
Product group Antibodies
Boster Bio
Anti-GR NR3C1 Rabbit Monoclonal AntibodyM00503
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetNR3C1
Western blot analysis of OPA1 using anti-OPA1 antibody (M00508) in HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified monoclonal antibody (Catalog # M00508) at 1:500~1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5
Product group Antibodies
Boster Bio
Anti-OPA1 Monoclonal AntibodyM00508
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetOPA1
Western blot analysis of IDH2 expression in MOLT4 cell lysate using anti-IDH2 antibody (M00510). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH2 antigen affinity purified polyclonal antibody (Catalog # M00510) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IDH2.
Product group Antibodies
Boster Bio
Anti-IDH2 Monoclonal AntibodyM00510
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetIDH2
Immunohistochemical analysis of paraffin-embedded human kidney, using CDK5 Antibody.
Product group Antibodies
Boster Bio
Anti-CDK5 Rabbit Monoclonal AntibodyM00511
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDK5
Western blot analysis of PON1 using anti-PON1 antibody (M00516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysate, Lane 2: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PON1 antigen affinity purified monoclonal antibody (Catalog # M00516-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-PON1 Antibody Picoband(r) (monoclonal, 6B1)M00516-1
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPon1
Sandwich ELISA - Recombinant mouse PON1 protein standard curve.
Product group Antibodies
Boster Bio
Anti-PON1 Antibody (monoclonal, 11H2)M00516-2
ApplicationsELISA
ReactivityMouse, Rat
TargetPon1
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