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CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immunoprecipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse antisheep monoclonal antibody.
Product group Antibodies
OriGene
Acid Phosphatase (ACP1) Sheep Polyclonal AntibodyAP05058PU-N
ApplicationsImmunoPrecipitation, Western Blot
ReactivityBovine, Human, Mouse, Rat
TargetACP1
  • SizePrice
Product group Antibodies
OriGene
Calcineurin A (PPP3CA) Sheep Polyclonal AntibodyAP05059PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetPPP3CA
  • SizePrice
Eph receptor B1 (EPHB1) (EXT Region) Sheep Polyclonal Antibody
Product group Antibodies
OriGene
Eph receptor B1 (EPHB1) (EXT Region) Sheep Polyclonal AntibodyAP05060PU-N
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetEPHB1
  • SizePrice
Product group Antibodies
OriGene
Eph receptor B1 (EPHB1) (SAM Region) Sheep Polyclonal AntibodyAP05061PU-N
ApplicationsImmunoPrecipitation
ReactivityHuman
TargetEPHB1
  • SizePrice
Eph receptor B1 (EPHB1) (Cytopl. Dom.) Sheep Polyclonal Antibody
Product group Antibodies
OriGene
Eph receptor B1 (EPHB1) (Cytopl. Dom.) Sheep Polyclonal AntibodyAP05062PU-N
ApplicationsImmunoPrecipitation
ReactivityHuman
TargetEPHB1
  • SizePrice
HEK293 cells were transfected with either emply expression vector (pSRa) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 microl/300 ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 microl/300 ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 microl/300 ug lysate). DPH or SHD anti Vav2 antisera (2.5 microl/300 ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.
Product group Antibodies
OriGene
VAV2 (SHD Region) Sheep Polyclonal AntibodyAP05063PU-N
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetVAV2
  • SizePrice
HEK293 cells were transfected with either emply expression vector (pSR_) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 ug lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 microl/300ug lysate). DPH or SHD alpha Vav2 antiserum (2.5 microl/300ug lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 microl/300ug lysate). DPH or SHD anti Vav2 antisera (2.5microl/300ug lysate). Wersterm blot Ab - SHD anti Vav2 antibody 1:10000.
Product group Antibodies
OriGene
VAV2 (DPH Region) Sheep Polyclonal AntibodyAP05064PU-N
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetVAV2
  • SizePrice
Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/5%NFDM, probed with 5 g/ml of pre-immune serum 5 g of SSeCKS antibod
Product group Antibodies
OriGene
AKAP12 / SSeCKS Sheep Polyclonal AntibodyAP05065PU-N
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityBovine, Rat
  • SizePrice
Product group Antibodies
OriGene
MTGR1 (CBFA2T2) Sheep Polyclonal AntibodyAP05066PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetCBFA2T2
  • SizePrice
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Product group Antibodies
OriGene
Nephronophthisis (NPHP1) Rabbit Polyclonal AntibodyAP05067PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse
TargetNPHP1
  • SizePrice
FLI1 Sheep Polyclonal Antibody
Product group Antibodies
OriGene
FLI1 Sheep Polyclonal AntibodyAP05068PU-N
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetFLI1
  • SizePrice
LEF1 Sheep Polyclonal Antibody
Product group Antibodies
OriGene
LEF1 Sheep Polyclonal AntibodyAP05069PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetLEF1
  • SizePrice
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