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Western blot analysis of PROX1 expression in HepG2 cell lysate (M01985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 monoclonal antibody (Catalog # M01985) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROX1
Product group Antibodies
Boster Bio
Anti-PROX1 Rabbit Monoclonal AntibodyM01985
ApplicationsWestern Blot
ReactivityHuman
TargetPROX1
Western blot analysis of GAB1 expression in HEK293 cell lysate.
Product group Antibodies
Boster Bio
Anti-GAB1 Monoclonal AntibodyM01989
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetGAB1
Western blot analysis of GAD67 expression in (1) Jurkat cell lysate; (2) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-GAD67 GAD1 Rabbit Monoclonal AntibodyM02002
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetGAD1
Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody.
Product group Antibodies
Boster Bio
Anti-Actin ACTA1 Rabbit Monoclonal AntibodyM02014-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetACTA1
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate (M02014-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1
Product group Antibodies
Boster Bio
Anti-Actin ACTA1 Rabbit Monoclonal AntibodyM02014-2
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetACTA1
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Product group Antibodies
Boster Bio
Anti-Actin ACTA1 Rabbit Monoclonal AntibodyM02014-3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetACTA1
Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody(M02014-4) ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-4)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal AntibodyM02014-4
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetACTA1
Western blot analysis of Mad2L1 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-NuMA NUMA1 Monoclonal AntibodyM02018-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNUMA1
Western blot analysis of MLANA expression in Human melanoma tissue lysate (M02033-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLANA monoclonal antibody (Catalog # M02033-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLANA
Product group Antibodies
Boster Bio
Anti-MLANA/Mart 1 Rabbit Monoclonal AntibodyM02033-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMLANA
Western blot analysis of SERPINF1 expression in K562 cell lysate using anti-SERPINF1 antibody (M02034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINF1 antigen affinity purified polyclonal antibody (Catalog # M02034) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SERPINF1.
Product group Antibodies
Boster Bio
Anti-PEDF Monoclonal AntibodyM02034
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetSERPINF1
Western blot analysis of Desmoglein 2 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Desmoglein 2 Monoclonal AntibodyM02035
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDSG2
Western blot analysis of c-Jun expression in NIH/3T3 cell lysates (M02038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun monoclonal antibody (Catalog # M02038) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-c-Jun Rabbit Monoclonal AntibodyM02038
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUN
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