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Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2-PE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Figure 1. Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-RPN1 Antibody Picoband(r)A05063-2
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
Product group Antibodies
Boster Bio
Anti-RPN1 AntibodyA05063
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetRPN1
  • SizePrice
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