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Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-RTCB Antibody Picoband(r)A05076-2-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRTCB
  • SizePrice
Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-RTCB Antibody Picoband(r)A05076-2-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRTCB
  • SizePrice
Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-RTCB Antibody Picoband(r)A05076-2-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRTCB
  • SizePrice
Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-RTCB Antibody Picoband(r)A05076-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRTCB
  • SizePrice
Figure 1. Western blotting validation for Anti-PRKAB2 Antibody A05077 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
Anti-PRKAB2 AntibodyA05077
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetPRKAB2
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Synaptotagmin-7 SYT7 AntibodyA05078
ApplicationsWestern Blot, ELISA
TargetSYT7
  • SizePrice
Western blot analysis of Aldh3A2 in mouse liver lysate with Aldh3A2 antibody at (A) 1 and (B) 2 microg/mL.
Product group Antibodies
Boster Bio
Anti-Fatty aldehyde dehydrogenase Aldh3A2 AntibodyA05079
ApplicationsWestern Blot, ELISA
TargetALDH3A2
  • SizePrice
Product group Antibodies
Boster Bio
Anti-R-spondin-2 RSPO2 AntibodyA05081
ApplicationsWestern Blot, ELISA
TargetRSPO2
  • SizePrice
Product group Antibodies
Boster Bio
Anti-C5ARL C5AR2 AntibodyA05083
ApplicationsWestern Blot, ELISA
TargetC5AR2
  • SizePrice
Immunohistochemistry of TAB3 in human kidney tissue with TAB3 antibody at 5 microg/mL.
Product group Antibodies
Boster Bio
Anti-TAB3 AntibodyA05084-1
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetTAB3
  • SizePrice
Product group Antibodies
Boster Bio
Anti-TAB3 AntibodyA05084-2
ApplicationsWestern Blot, ELISA
TargetTAB3
  • SizePrice
Figure 1. Western blot analysis of TAB3 using anti-TAB3 antibody (A05084-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified polyclonal antibody (Catalog # A05084-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.
Product group Antibodies
Boster Bio
Anti-TAB3 Antibody Picoband(r)A05084-3-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetTAB3
  • SizePrice
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