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Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-3-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-3-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-3-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-3-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-3-PE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
References
Boster Bio
Anti-TNF Receptor I/TNFRSF1A AntibodyA00294-3
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
Figure 1. Western blot analysis of TNF Receptor I/TNFRSF1A using anti-TNF Receptor I/TNFRSF1A antibody (A00294-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I/TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I/TNFRSF1A at approximately 60-70 kDa. The expected band size for TNF Receptor I/TNFRSF1A is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband(r)A00294-4-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetTNFRSF1A
  • SizePrice
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