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Western blot analysis of Insulin Receptor expression in HeLa cell lysate (M00447). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INSR monoclonal antibody (Catalog # M00447) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INSR
Product group Antibodies
Boster Bio
Anti-Insulin Receptor Rabbit Monoclonal AntibodyM00447
Western blot analysis of PPAR gamma expression in (1) HeLa cell lysate; (2) PC-3 cell lysate; (3) THP-1 cell lysate.
Product group Antibodies
Boster Bio
Anti-PPAR gamma Rabbit Monoclonal AntibodyM00449
Western blot analysis of Lipocalin-2 expression in SW480 cell lysate (M00452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCN2 monoclonal antibody (Catalog # M00452) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCN2
Product group Antibodies
Boster Bio
Anti-Lipocalin-2 Rabbit Monoclonal AntibodyM00452
Western blot analysis of PAK1 expression in A431 cell lysate using anti-PAK1 antibody (M00454). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # M00454) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PAK1.
Product group Antibodies
Boster Bio
Anti-PAK1 Monoclonal AntibodyM00454
Western blot analysis of beta 2 Microglobulin expression in U937 cell lysate.
Product group Antibodies
Boster Bio
Anti-beta 2 Microglobulin Rabbit Monoclonal AntibodyM00456-1
Western blot analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2).
Product group Antibodies
Boster Bio
Anti-Beta 2 Microglobulin Antibody (monoclonal, 2H10)M00456-2
Western blot analysis of ABCG2 expression in 293T cell lysate.
Product group Antibodies
Boster Bio
Anti-ABCG2/Bcrp Rabbit Monoclonal AntibodyM00457
IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD18 Antibody (monoclonal, 1A3/2A10)M00458-1
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CD18 DyLight 550 conjugated Antibody(monoclonal, 1A3/2A10)M00458-Dyl550
Western blot analysis of CD18 expression in K562 cell lysate (M00458). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB2 monoclonal antibody (Catalog # M00458) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB2
Product group Antibodies
Boster Bio
Anti-CD18 Rabbit Monoclonal AntibodyM00458
Product group Lysates / Extracts
Boster Bio
HeLa Cell Lysate (Whole Cell)LYSATE0001
Product group Lysates / Extracts
Boster Bio
Rat Brain Tissue LysateLYSATE0002
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