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Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SMMC-7721 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Product group Antibodies
Boster Bio
Anti-CRM1 XPO1 Antibody Picoband(r) (monoclonal, 5G3)M01180-FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetXPO1
  • SizePrice
Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SMMC-7721 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Product group Antibodies
Boster Bio
Anti-CRM1 XPO1 Antibody Picoband(r) (monoclonal, 5G3)M01180-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetXPO1
  • SizePrice
Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SMMC-7721 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Product group Antibodies
Boster Bio
Anti-CRM1 XPO1 Antibody Picoband(r) (monoclonal, 5G3)M01180-IFLUOR647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetXPO1
  • SizePrice
Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SMMC-7721 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Product group Antibodies
Boster Bio
Anti-CRM1 XPO1 Antibody Picoband(r) (monoclonal, 5G3)M01180-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetXPO1
  • SizePrice
Figure 1. Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SMMC-7721 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.
Product group Antibodies
Boster Bio
Anti-CRM1 XPO1 Antibody Picoband(r) (monoclonal, 5G3)M01180
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetXPO1
  • SizePrice
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-PIM1 Rabbit Monoclonal AntibodyM01184-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPIM1
  • SizePrice
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-PIM1 Rabbit Monoclonal AntibodyM01184
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPIM1
  • SizePrice
Western blot analysis of FOXL2 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-FOXL2 Rabbit Monoclonal AntibodyM01185-30UL
ApplicationsWestern Blot
ReactivityHuman
TargetFOXL2
  • SizePrice
Western blot analysis of FOXL2 expression in Jurkat cell lysate.
Product group Antibodies
References
Boster Bio
Anti-FOXL2 Rabbit Monoclonal AntibodyM01185
ApplicationsWestern Blot
ReactivityHuman
TargetFOXL2
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPB (1:2000).
Product group Antibodies
Boster Bio
Anti-BNP (NPPB) Mouse Monoclonal Antibody [Clone ID: OTI11G4]M01186-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetNPPB
  • SizePrice
Western blot analysis of BNP expression in human plasma lysate.
Product group Antibodies
Boster Bio
Anti-BNP Rabbit Monoclonal AntibodyM01186-2-30UL
ApplicationsWestern Blot
ReactivityHuman
TargetNPPB
  • SizePrice
Western blot analysis of BNP expression in human plasma lysate.
Product group Antibodies
References
Boster Bio
Anti-BNP Rabbit Monoclonal AntibodyM01186-2
ApplicationsWestern Blot
ReactivityHuman
TargetNPPB
  • SizePrice
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