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IHC analysis of BRG1 using anti-BRG1 antibody (A00223-1).
Product group Antibodies
Boster Bio
IHC analysis of AHR using anti-AHR antibody (A00225-2). AHR was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1).
Product group Antibodies
Boster Bio
Product group Antibodies
Boster Bio
Western blot analysis of IL4 using anti-IL4 antibody (A00230).
Product group Antibodies
Boster Bio
Western blot analysis of Hemoglobin using anti-Hemoglobin antibody (A00233-1).
Product group Antibodies
Boster Bio
Western blot analysis of DDX58 using anti-DDX58 antibody (A00244-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: mouse spleen tissue lysate, Lane 4: mouse thymus tissue lysate, Lane 5: mouse NIH3T3 whole Cell lysate, Lane 6: human Jurkat whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody (Catalog # A00244-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 116KD. The expected band size for DDX58 is at 106KD.
Product group Antibodies
Boster Bio
IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (A00248-1).
Product group Antibodies
Boster Bio
Flow Cytometry analysis of A549 cells using anti-Human CD147/Emmprin antibody (A00248-Dyl488).
Product group Antibodies
Boster Bio
Boster Kit Box
Product group Antibodies
Boster Bio
IHC analysis of Crp using anti-Crp antibody (A00249-1).
Product group Antibodies
Boster Bio
IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-2). C Reactive Protein was detected in paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-C Reactive Protein Antibody (A00249-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio