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ProductsBack/Anti-PIM1 Rabbit Monoclonal Antibody
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

Anti-PIM1 Rabbit Monoclonal Antibody

M01184
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPIM1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PIM1 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ECB-16
  • Gene ID5292
  • Target name
    PIM1
  • Target description
    Pim-1 proto-oncogene, serine/threonine kinase
  • Target synonyms
    PIM, serine/threonine-protein kinase pim-1, Oncogene PIM1, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11309
  • Protein Name
    Serine/threonine-protein kinase pim-1
  • Scientific Description
    Boster Bio Anti-PIM1 Rabbit Monoclonal Antibody catalog # M01184. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • PIM1 polymorphism and PIM1 expression as predisposing factors of esophageal squamous cell carcinoma in the Asian population.
    Read more

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of PIM1 using anti-PIM1 antibody (PB9315). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM1 antigen affinity purified polyclonal antibody (Catalog # PB9315) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIM1 at approximately 44KD. The expected band size for PIM1 is at 44KD.
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