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Sandwich ELISA - Recombinant mouse Lipocalin 2 protein standard curve.
Product group Antibodies
Boster Bio
Anti-Lipocalin 2 AntibodyA00452
Western blot analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (A00456-1).
Product group Antibodies
Boster Bio
Anti-Beta 2 Microglobulin AntibodyA00456-1
Western blot analysis of CAD using anti-CAD antibody (A00463-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.
Product group Antibodies
Boster Bio
Anti-CAD/CAD AntibodyA00463-1
Western blot analysis of COMT using anti-COMT antibody (A00464).
Product group Antibodies
Boster Bio
Anti-COMT AntibodyA00464
Western blot analysis of TRAIL using anti-TRAIL antibody (A00466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 35KD. The expected band size for TRAIL is at 33KD.
Product group Antibodies
Boster Bio
Anti-TRAIL AntibodyA00466-1
Western blot analysis of TRAIL using anti-TRAIL antibody (A00466).
Product group Antibodies
Boster Bio
Anti-TRAIL AntibodyA00466
Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Product group Antibodies
Boster Bio
Anti-Galectin 1 AntibodyA00470-1
Sandwich ELISA - Recombinant mouse Galectin 1 protein standard curve.
Product group Antibodies
Boster Bio
Anti-Galectin 1 AntibodyA00470-2
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Galectin 1 Biotinylated AntibodyA00470-Biotin
Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse ovary tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: rat gaster tissue lysates, Lane 8: rat testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Product group Antibodies
Boster Bio
Anti-Galectin 1 AntibodyA00470
Flow Cytometry analysis of K562 cells using anti-Human CYP1A1 antibody (A00471-Dyl488).
Product group Antibodies
Boster Bio
Anti-Human CYP1A1 DyLight 488 conjugated AntibodyA00471-Dyl488
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CYP1A1 DyLight 550 conjugated AntibodyA00471-Dyl550
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