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Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.
Product group Antibodies
Boster Bio
Anti-Cyclin D1 Rabbit Monoclonal AntibodyM00149-1
Western blot analysis of SHP2 expression in A431 cell lysate (M00150-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # M00150-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11
Product group Antibodies
Boster Bio
Anti-SHP2 Rabbit Monoclonal AntibodyM00150-1
Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
Product group Antibodies
Boster Bio
Anti-SHP2/PTPN11 Antibody (monoclonal, 2E6)M00150-2
Western blot analysis of SHP2 expression in Jurkat cell lysate (M00150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # M00150) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11
Product group Antibodies
Boster Bio
Anti-SHP2 Rabbit Monoclonal AntibodyM00150
Immunohistochemical analysis of paraffin-embedded mouse spleen, using FAK Antibody(M00151) PTK2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTK2 Antibody (M00151)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-FAK Rabbit Monoclonal AntibodyM00151
Immunohistochemical analysis of paraffin-embedded rat testis, using Nanog Antibody.
Product group Antibodies
Boster Bio
Anti-Nanog Rabbit Monoclonal AntibodyM00153
Western blot analysis of CD19 expression in (1) Jurkat cell lysate; (2) Rat spleen lysate.
Product group Antibodies
Boster Bio
Anti-CD19 Rabbit Monoclonal AntibodyM00154-1
Western blot analysis of CD19 expression in Ramos cell lysate.
Product group Antibodies
Boster Bio
Anti-CD19 Rabbit Monoclonal AntibodyM00154
Immunohistochemical analysis of paraffin-embedded human tonsil, using CCR2/CKR2.
Product group Antibodies
Boster Bio
Anti-CCR2/CKR2M00158
Western blot analysis of CDK4 expression in HeLa cell lysate (M00159-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody (Catalog # M00159-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4
Product group Antibodies
Boster Bio
Anti-CDK4 Rabbit Monoclonal AntibodyM00159-1
Western blot analysis of CDK4 expression in HeLa cell lysate (M00159). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 monoclonal antibody (Catalog # M00159) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4
Product group Antibodies
Boster Bio
Anti-CDK4 Rabbit Monoclonal AntibodyM00159
Western blot analysis of PRC1 expression in HeLa cell lysate (M00160). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 monoclonal antibody (Catalog # M00160) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRC1
Product group Antibodies
Boster Bio
Anti-PRC1 Rabbit Monoclonal AntibodyM00160
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