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Anti-MAPK8/9 antibody, PA1407, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Thymus Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: JURKAT Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: CEM Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetMAPK8
Anti-Podoplanin/gp36 antibody, PA1411, Western blotting All lanes: Anti Podoplanin/gp36 (PA1411) at 0.5ug/ml Lane 1: SMMC Whole Cell Lysate at 40ug Lane 2: 293T Whole Cell Lysate at 40ug Lane 3: HELA Whole Cell Lysate at 40ug Predicted bind size: 36KD Observed bind size: 36KD
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Monkey
TargetPDPN
Anti-Progesterone Receptor antibody, PA1413, Western blotting WB: HELA Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human
TargetPGR
Anti-Pleiotrophin antibody, PA1414, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HT1080 Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPTN
Anti-NDRG1 antibody, PA1416, Western blotting Lane 1: SMMC Nucleus Cell Lysate Lane 2: SMMC Cytoplasm Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Equine, Human, Rabbit
TargetNDRG1
Western blot analysis of Thrombospondin 2/THBS2 using anti-Thrombospondin 2/THBS2 antibody (PA1417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2/THBS2 antigen affinity purified polyclonal antibody (Catalog # PA1417) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombospondin 2/THBS2 at approximately 160-190KD. The expected band size for Thrombospondin 2/THBS2 is at 130KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human
TargetTHBS2
Anti-Ubiquitin antibody, PA1420, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: Human MCF-7 Cell Lysate Lane 3: MM231 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetUBB
Western blot analysis of Fetuin A using anti-Fetuin A antibody (PA1421).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetAHSG
Western blot analysis of LGALS1 using anti-LGALS1 antibody (PA1422).
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetLGALS1
Anti-IL-3 antibody, PA1423, Western blotting Lane 1: Recombinant Human IL-3 Protein 10ng Lane 2: Recombinant Human IL-3 Protein 5ng Lane 3: Recombinant Human IL-3 Protein 2.5ng
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetIL3
Anti-IL-4 antibody, PA1424, Western blotting Lane 1: Recombinant Human IL-4 Protein 10ng Lane 2: Recombinant Human IL-4 Protein 5ng Lane 3: Recombinant Human IL-4 Protein 2.5ng
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetIL4
IHC analysis of BCL2L2 using anti-BCL2L2 antibody (PA1426-1). BCL2L2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BCL2L2 Antibody (PA1426-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetBCL2L2