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MATR3 antibody

10R-1442
Biosynth
Product group Antibodies
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    MATR3 antibody
  • Delivery Days Customer
    11
  • Applications Supplier
    Dot Blot, ICC, WB
  • Certification
    Research Use Only
  • Clone ID
    2539C3a
  • Concentration
    100 ug/ml
  • Reactivity Supplier
    Human, Mouse, Rat. Other species not studied
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MATR3 using anti-MATR3 antibody (A02931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATR3 antigen affinity purified polyclonal antibody (Catalog # A02931-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATR3 at approximately 125 kDa. The expected band size for MATR3 is at 95 kDa.
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