DNA-binding activity of MAX was measured in OriGene over-expression lysate LY408018 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing MAX-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed MAX is biologically active in the lysate. Overexpression cell lysates are prepared from HEK293T cells transfected with RC206812 using transfection reagent MegaTran 2.0 (Cat# TT210002).