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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA883444LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA883444LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA883444LA01HU at dilution of 1:100

MBD1 Antibody

CSB-PA883444LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMBD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MBD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4152
  • Target name
    MBD1
  • Target description
    methyl-CpG binding domain protein 1
  • Target synonyms
    CXXC3; CXXC-type zinc finger protein 3; methyl-CpG-binding domain protein 1; PCM1; protein containing methyl-CpG-binding domain 1; RFT; the regulator of fibroblast growth factor 2 (FGF-2) transcription
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIS9
  • Protein Name
    Methyl-CpG-binding domain protein 1
  • Scientific Description
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone Lys-9 trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBD1 using anti-MBD1 antibody (A02336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified polyclonal antibody (Catalog # A02336-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD1 at approximately 67KD. The expected band size for MBD1 is at 67KD.
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