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ProductsBack/MBL2 antibody [4J25]
WB analysis of HEK293 expressing human MBL using GTX52919 MBL2 antibody [4J25].
WB analysis of HEK293 expressing human MBL using GTX52919 MBL2 antibody [4J25].
WB analysis of HEK293 expressing human MBL using GTX52919 MBL2 antibody [4J25].

MBL2 antibody [4J25]

GTX52919
GeneTex
ApplicationsWestern Blot, Neutralisation/Blocking
DownloadDatasheet
Product group Antibodies
TargetMBL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    MBL2 antibody [4J25]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4J25
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID4153
  • Target name
    MBL2
  • Target description
    mannose binding lectin 2
  • Target synonyms
    COLEC1; collectin-1; HSMBPC; mannan-binding lectin; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding protein C; MBL; MBL2D; MBP; MBP1; MBP-C; MBPD
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDP11226
  • Protein Name
    Mannose-binding protein C
  • Scientific Description
    This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
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