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ProductsBack/MBL2 / Mannose Binding Protein Antibody (clone 14D12)

MBL2 / Mannose Binding Protein Antibody (clone 14D12)

LS-C139870
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetMBL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MBL2 / Mannose Binding Protein Antibody (clone 14D12)
  • Delivery Days Customer
    23
  • Application Supplier Note
    For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody).. IF, IHC, IHC-Fr, WB For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody).
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14D12
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4153
  • Target name
    MBL2
  • Target description
    mannose binding lectin 2
  • Target synonyms
    COLEC1; collectin-1; HSMBPC; mannan-binding lectin; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); mannose-binding protein C; MBL; MBL2D; MBP; MBP1; MBP-C; MBPD
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
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