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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA889088LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA889088LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA889088LA01HU at dilution of 1:100

MBNL1 Antibody

CSB-PA889088LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMBNL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MBNL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4154
  • Target name
    MBNL1
  • Target description
    muscleblind like splicing regulator 1
  • Target synonyms
    EXP; MBNL; muscleblind-like; muscleblind-like protein 1; triplet-expansion RNA-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR56
  • Protein Name
    Muscleblind-like protein 1
  • Scientific Description
    Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5-YGCU(U/G)Y-3consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBNL1 antigen affinity purified polyclonal antibody (Catalog # A02309-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBNL1 at approximately 40 kDa. The expected band size for MBNL1 is at 42 kDa.
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