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Western Blot analysis of HeLa cells using MC2-R Polyclonal Antibody
Western Blot analysis of HeLa cells using MC2-R Polyclonal Antibody
Western Blot analysis of HeLa cells using MC2-R Polyclonal Antibody

MC2R Antibody

CSB-PA003204
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetMC2R
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Overview

  • Supplier
    Cusabio
  • Product Name
    MC2R Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4158
  • Target name
    MC2R
  • Target description
    melanocortin 2 receptor
  • Target synonyms
    ACTH receptor; ACTHR; adrenocorticotropic hormone receptor; adrenocorticotropin receptor; corticotropin receptor; MC2 receptor; melanocortin 2 receptor (adrenocorticotropic hormone)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01718
  • Protein Name
    Adrenocorticotropic hormone receptor
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MC2 receptor using anti-MC2 receptor antibody (A02306-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC2 receptor antigen affinity purified polyclonal antibody (Catalog # A02306-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC2 receptor at approximately 39KD. The expected band size for MC2 receptor is at 34KD.
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