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MC4R Antibody (YA1645, Rabbit)

HY-P81900
MedChem Express
TargetMC4R
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MC4R Antibody (YA1645, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4160
  • Target name
    MC4R
  • Target description
    melanocortin 4 receptor
  • Target synonyms
    BMIQ20, melanocortin receptor 4, MC4-R, mutant melanocortin-4 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MC4R Antibody (YA1645) is a non-conjugated IgG antibody, targeting MC4R, with a predicted molecular weight of 37 kDa (observed band size: 37 kDa). MC4R Antibody (YA1645) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MC4R using anti-MC4R antibody (A00619-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC4R antigen affinity purified polyclonal antibody (Catalog # A00619-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC4R at approximately 37KD. The expected band size for MC4R is at 37KD.
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