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ProductsBack/MCAM / CD146 Antibody (clone OJ79c, FITC)

MCAM / CD146 Antibody (clone OJ79c, FITC)

LS-C44323
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetMCAM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MCAM / CD146 Antibody (clone OJ79c, FITC)
  • Delivery Days Customer
    14
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flo The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OJ79c
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID4162
  • Target name
    MCAM
  • Target description
    melanoma cell adhesion molecule
  • Target synonyms
    CD146; cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18; MelCAM; METCAM; MUC18; S-endo 1 endothelial-associated antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD146 using anti-CD146 antibody (RP1050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD146 antigen affinity purified polyclonal antibody (Catalog # RP1050) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120 kDa. The expected band size for CD146 is at 72 kDa.
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