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MCCC2 Antibody

CAB7990
Assay Genie
TargetMCCC2
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    MCCC2 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64087
  • Target name
    MCCC2
  • Target description
    methylcrotonyl-CoA carboxylase subunit 2
  • Target synonyms
    3-methylcrotonyl-CoA carboxylase 2; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta; biotin carboxylase; MCCase subunit beta; MCCB; MCCCbeta; methylcrotonoyl-CoA carboxylase 2 (beta); methylcrotonoyl-CoA carboxylase beta chain, mitochondrial; methylcrotonoyl-Coenzyme A carboxylase 2 (beta); non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase; testicular secretory protein Li 29
  • Host
    Rabbit
  • Protein IDQ9HCC0
  • Protein Name
    Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: MCCC2 antibody at 6.85microg/ml Lane 1: Mouse brain tissue Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 62, 58 kDa Observed band size: 62 kDa
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Lane 1: Mouse kidney lysates; Lane 2: DU145 cell lysates probed with MCCC2 Polyclonal Antibody, Unconjugated (bs-18719R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MCCC2 using anti-MCCC2 antibody (A06515-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCCC2 antigen affinity purified polyclonal antibody (Catalog # A06515-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCCC2 at approximately 61 kDa. The expected band size for MCCC2 is at 61 kDa.
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