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MCL1 Antibody

HY-P80508
MedChem Express
TargetMCL1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MCL1 Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4170
  • Target name
    MCL1
  • Target description
    MCL1 apoptosis regulator, BCL2 family member
  • Target synonyms
    BCL2 family apoptosis regulator; BCL2L3; bcl2-L-3; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl-1; MCL1, BCL2 family apoptosis regulator; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MCL1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 37 kDa, targeting to MCL1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MCL1 using anti-MCL1 antibody (A00712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat kisney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified polyclonal antibody (Catalog # A00712-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 40 kDa. The expected band size for MCL1 is at 28,37,40 kDa.
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