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ProductsBack/MCL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCL1 (Cat# RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1(Cat# TA500986). Positive lysates [LY411855] (100ug) and [LC411855] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCL1 (Cat# RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1(Cat# TA500986). Positive lysates [LY411855] (100ug) and [LC411855] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCL1 (Cat# RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1(Cat# TA500986). Positive lysates [LY411855] (100ug) and [LC411855] (20ug) can be purchased separately from OriGene.

MCL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]

CF500986
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetMCL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MCL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E11
  • Gene ID4170
  • Target name
    MCL1
  • Target description
    MCL1 apoptosis regulator, BCL2 family member
  • Target synonyms
    BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, induced myeloid leukemia cell differentiation protein Mcl-1, BCL2 family apoptosis regulator, MCL1, BCL2 family apoptosis regulator, bcl-2-like protein 3, bcl-2-related protein EAT/mcl1, myeloid cell leukemia 1, myeloid cell leukemia ES, myeloid cell leukemia sequence 1 (BCL2-related)
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ07820
  • Protein Name
    Induced myeloid leukemia cell differentiation protein Mcl-1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MCL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MCL1 using anti-MCL1 antibody (A00712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat kisney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified polyclonal antibody (Catalog # A00712-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 40 kDa. The expected band size for MCL1 is at 28,37,40 kDa.
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