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MCM3AP / GANP Antibody (aa1-50)

LS-C289826
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMCM3AP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MCM3AP / GANP Antibody (aa1-50)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:5000) IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8888
  • Target name
    MCM3AP
  • Target description
    minichromosome maintenance complex component 3 associated protein
  • Target synonyms
    80 kDa MCM3-associated protein; GANP; germinal center-associated nuclear protein; germinal-center associated nuclear protein; germinal-centre associated nuclear protein; MAP80; MCM3 acetylating protein; MCM3 acetyltransferase; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; PNRIID; SAC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GANP/MCM3AP using anti-GANP/MCM3AP antibody (A05445-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GANP/MCM3AP antigen affinity purified polyclonal antibody (Catalog # A05445-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GANP/MCM3AP at approximately 218 kDa. The expected band size for GANP/MCM3AP is at 218 kDa.
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