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Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500

MCM6 Polyclonal Antibody

E-AB-10451
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMCM6
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Overview

  • Supplier
    Elabscience
  • Product Name
    MCM6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4175
  • Target name
    MCM6
  • Target description
    minichromosome maintenance complex component 6
  • Target synonyms
    MCG40308, Mis5, P105MCM, DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), minichromosome maintenance deficient (mis5, S. pombe) 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14566
  • Protein Name
    DNA replication licensing factor MCM6
  • Scientific Description
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of MCM6 using anti-MCM6 antibody (A02755-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human MOLT-4 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat ovary tissue lysates, Lane 10: rat thymus tissue lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody (Catalog # A02755-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM6 at approximately 105 kDa. The expected band size for MCM6 is at 93 kDa.
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