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IHC-P analysis of mouse brain tissue using GTX37377 MCP3 antibody.
IHC-P analysis of mouse brain tissue using GTX37377 MCP3 antibody.
IHC-P analysis of mouse brain tissue using GTX37377 MCP3 antibody.

MCP3 antibody

GTX37377
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCcl7
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Overview

  • Supplier
    GeneTex
  • Product Name
    MCP3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:400-800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID20306
  • Target name
    Ccl7
  • Target description
    C-C motif chemokine ligand 7
  • Target synonyms
    MCP-3, Scya7, fic, marc, mcp3, C-C motif chemokine 7, RANTES/sis homolog, chemokine (C-C motif) ligand 7, intercrine/chemokine MARC, monocyte chemoattractant protein 3, monocyte chemotactic protein 3, small-inducible cytokine A7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03366
  • Protein Name
    C-C motif chemokine 7
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of MCP3 using anti-MCP3 antibody (PB9029). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Mouse MCP3 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP3 antigen affinity purified polyclonal antibody (Catalog # PB9029) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP3 at approximately 36KD. The expected band size for MCP3 is at 36KD.
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