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Western blot analysis of lysates from HeLa cells using MCU Rabbit pAb (TA378411) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Western blot analysis of lysates from HeLa cells using MCU Rabbit pAb (TA378411) at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25 μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 30s.
Western blot analysis of lysates from HeLa cells using MCU Rabbit pAb (TA378411) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

MCU Rabbit Polyclonal Antibody

TA378411
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMCU
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MCU Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID90550
  • Target name
    MCU
  • Target description
    mitochondrial calcium uniporter
  • Target synonyms
    C10orf42, CCDC109A, HsMCU, calcium uniporter protein, mitochondrial, coiled-coil domain-containing protein 109A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NE86
  • Protein Name
    Calcium uniporter protein, mitochondrial
  • Scientific Description
    MCU Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MCU using anti-MCU antibody (A00685-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat PC-12 whole cell lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCU antigen affinity purified polyclonal antibody (Catalog # A00685-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCU at approximately 34KD. The expected band size for MCU is at 34KD.
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