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Western Blot Positive WB detected in: Rat heart tissue All lanes: MECR antibody at 3.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 33 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: MECR antibody at 3.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 33 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: MECR antibody at 3.3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 33 kDa Observed band size: 41 kDa

MECR Antibody

CSB-PA887132LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMECR
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Overview

  • Supplier
    Cusabio
  • Product Name
    MECR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51102
  • Target name
    MECR
  • Target description
    mitochondrial trans-2-enoyl-CoA reductase
  • Target synonyms
    2-enoyl thioester reductase; CGI-63; DYTOABG; enoyl-[acyl-carrier-protein] reductase, mitochondrial; ETR1; FASN2B; homolog of yeast 2-enoyl thioester reductase; mitochondrial 2-enoyl thioester reductase; NRBF1; nuclear receptor binding factor 1; trans-2-enoyl-CoA reductase, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV79
  • Protein Name
    Enoyl-[acyl-carrier-protein] reductase, mitochondrial
  • Scientific Description
    Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MECR using anti-MECR antibody (A08189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MECR antigen affinity purified polyclonal antibody (Catalog # A08189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MECR at approximately 38 kDa. The expected band size for MECR is at38 kDa.
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