More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Goat anti-MED12 (aa1981-1994) Antibody

Goat anti-MED12 (aa1981-1994) Antibody

ORB107655
Biorbyt
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetMED12
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-MED12 (aa1981-1994) Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    WB: Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5microg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat
  • Applications
    ELISA
  • Applications Supplier
    ELISA: 1:32000, WB: 0.5 μg/ml ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9968
  • Target name
    MED12
  • Target description
    mediator complex subunit 12
  • Target synonyms
    ARC240, CAGH45, FGS1, HDKR, HOPA, Kto, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230, mediator of RNA polymerase II transcription subunit 12, CAG repeat protein 45, Kohtalo homolog, OPA-containing protein, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA polymerase II transcription, subunit 12 homolog, putative mediator subunit 12, thyroid hormone receptor-associated protein complex 230 kDa component, thyroid hormone receptor-associated protein, 230 kDa subunit, trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit), trinucleotide repeat-containing gene 11 protein
  • Host
    Goat
  • Scientific Description
    Goat polyclonal to MED12
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Mouse spleen lysates probed with MED12/Trap230 Polyclonal Antibody, Unconjugated (bs-18763R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss
MED12/Trap230 Polyclonal AntibodyBS-18763R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetMED12
  • SizePrice
Anti-MED12 Antibody
Product group Antibodies
Antibodies.com
Anti-MED12 AntibodyA100662
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Immunohistochemical staining of human cerebral cortex, colon, spleen and testis using Anti-MED12 antibody HPA003184 (A) shows similar protein distribution across tissues to independent antibody HPA003185 (B).
Product group Antibodies
Atlas Antibodies
Anti-MED12 AntibodyHPA003184
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetMED12
  • SizePrice
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56106 MED12 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
Product group Antibodies
GeneTex
MED12 antibodyGTX56106
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetMED12
  • SizePrice
Product group Antibodies
Cusabio
MED12 AntibodyCSB-PA080112
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMED12
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MED12 (aa1981-1994) AntibodyEB10987
ApplicationsELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetMED12
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MED12 AntibodyLS-C406637
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMED12
  • SizePrice
Figure 1. Western blot analysis of MED12 using anti-MED12 antibody (A00828-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED12 antigen affinity purified polyclonal antibody (Catalog # A00828-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED12 at approximately 260 kDa. The expected band size for MED12 is at 260 kDa.
Product group Antibodies
Boster Bio
Anti-MED12 Antibody Picoband(r)A00828-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMED12
  • SizePrice