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ProductsBack/MED15 / ARC105 Antibody (aa393-679, clone OTI1H5)

MED15 / ARC105 Antibody (aa393-679, clone OTI1H5)

LS-C340416
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMED15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MED15 / ARC105 Antibody (aa393-679, clone OTI1H5)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1H5
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51586
  • Target name
    MED15
  • Target description
    mediator complex subunit 15
  • Target synonyms
    activator-recruited cofactor 105 kDa component; activator-recruited cofactor, 105-kD; ARC105; CAG7A; CTG repeat protein 7a; CTG7A; mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; PCQAP; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1; TIG-1; TNRC7; TPA inducible gene-1; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; trinucleotide repeat-containing gene 7 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MED15 using anti-MED15 antibody (A03568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: huamn K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED15 antigen affinity purified polyclonal antibody (Catalog # A03568-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED15 at approximately 97 kDa. The expected band size for MED15 is at 87 kDa.
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