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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA862046LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA862046LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA862046LA01HU at dilution of 1:100

MED20 Antibody

CSB-PA862046LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMED20
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Overview

  • Supplier
    Cusabio
  • Product Name
    MED20 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9477
  • Target name
    MED20
  • Target description
    mediator complex subunit 20
  • Target synonyms
    mediator of RNA polymerase II transcription subunit 20; PRO0213; SRB2; Trf (TATA binding protein-related factor)-proximal homolog; TRFP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H944
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 20
  • Scientific Description
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRFP / MED20 using anti-TRFP / MED20 antibody (A09407-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRFP / MED20 antigen affinity purified polyclonal antibody (Catalog # A09407-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRFP / MED20 at approximately 23KD. The expected band size for TRFP / MED20 is at 23KD.
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