ChIP analysis of HCT116 cells using GTX49103 MED25 antibody. Use 0.5microg antibody GTX49103 MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide
ChIP analysis of HCT116 cells using GTX49103 MED25 antibody. Use 0.5microg antibody GTX49103 MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide
MED25 antibody, N-term
GTX49103
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetMED25
Overview
- SupplierGeneTex
- Product NameMED25 antibody, N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID81857
- Target nameMED25
- Target descriptionmediator complex subunit 25
- Target synonymsACID1; activator interaction domain-containing protein 1; activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit; ARC92; BVSYS; CMT2B2; mediator of RNA polymerase II transcription subunit 25; mediator of RNA polymerase II transcription, subunit 25 homolog; P78; prostate-derived protein 78 kDa; PTOV2; TCBAP0758
- HostRabbit
- IsotypeIgG
- Protein IDQ71SY5
- Protein NameMediator of RNA polymerase II transcription subunit 25
- Scientific DescriptionThis gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203